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KMID : 0939920090410010036
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대한암학회지
2009 Volume.41 No. 1 p.36 ~ p.44
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Proapoptotic Ginsenosides CompoundK and Rh2Enhance Fas-induced Cell Death of Human AstrocytomaCells Through Distinct Apoptotic Signaling Pathways
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Choi Kyung-Sun
Choi Chul-Hee
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Abstract
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Purpose:Malignant astrocytomas are among the commonest primary brain tumors and they have agrave prognosis, and so there is an urgent need to develop effective treatment. In this study,we investigated the molecular mechanisms that are responsible for the anti-tumor effect ofginsenosides on human astrocytoma cells.
Materials and Methods:Wetested 13 different ginsenosides for their anti-tumor effect on human malignantastrocytoma cells in conjunction with Fas stimulation. In addition, the cell signaling pathwayswere explored by using pharmacological inhibitors and performing immunoblot analysis.DCF-DA staining and antioxidant experiments were performed to investigate the role ofreactive oxygen species as one of the apoptosis-inducing mechanisms.
Results:Among the 13 different ginsenoside metabolites, compound K and Rh2induced apoptotic celldeath of the astrocytoma cells in a caspase- and p38 MAPK-dependent manner, yet thesame treatment had no cytotoxic effect on the primary cultured human astrocytes. Combinedtreatment with ginsenosides and Fas ligand showed a synergistic cytotoxic effect, which wasmediated by the reduction of intracellular reactive oxygen species.
Conclusion:These results suggest that ginsenoside metabolites in combination with Fas ligand mayprovide a new strategy to treat malignant astrocytomas, which are tumors that are quiteresistant to conventional anti-cancer treatment.
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KEYWORD
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Apoptosis, Ginsenoside, Fas, Reactive oxygen species, Astrocytoma
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